Figure 4.

(A) Agarose gel electrophoresis analysis of amplicons generated by multiplex RT-PCR from RNA isolated from a nasopharyngeal swab taken from patients who had COVID-19 and diagnosed positive for SARS-CoV-2 by a laboratory-based test. Primer pairs are indicated above each amplicon and exemplar data from two patients (numbers 36 and 37) are shown. Note that amplicons from multiplex pool 1, for patient 36, is shown to the left as these were run on a separate gel. Also shown are negative controls and a positive control using RNA isolated from SARS-CoV-2 infected cells. (B,C) The amplicon products were purified, barcoded and sequenced on a single flow cell using an Oxford Nanopore MinION. Shown are the number of reads that map (y-axis) to each amplicon across the SARS-CoV-2 genome from 5′ to 3′ (x-axis).