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. 2020 Oct 15;13(10):314. doi: 10.3390/ph13100314

Figure 6.

Figure 6

Confocal laser scanning microscopy imaging of N2a695 cells treated with CS-SA/pre-miR-29b-fluorescein isothiocyanate isomer I (FITC) (35 N/P ratio) and CS-SA-Lf/pre-miR-29b-FITC (35 N/P ratio) polyplexes after different periods of incubation (0.5, 1, 1.5, and 2 h). The fluorescence signals were collected by Laser Scanning Confocal Microscopy (LSCM) with three channels: blue fluorescence from nuclei stained of cells with Hoechst 33342® (blue), green fluorescence from FITC labeled pre-miR-29b, and the merged images of three channels. Representative immunostaining data showing most of the FITC-labelled CS-SA-Lf/pre-miR-29b polyplexes localized in the cytoplasm. Scale bars 50 μm. The values are relative to the average FITC fluorescence intensity.