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. 2020 Oct 15;9(10):1000. doi: 10.3390/antiox9101000

Figure 7.

Figure 7

Dose-responses of Aβ to human retinal pigment epithelial (RPE) cell toxicity (A), temporal induction of tight junction markers of ZO-1 and occludin-1 by Aβ (B) and effects of eucalyptol on cellular induction of ZO-1 and occludin-1 and secretion of MMP-2 and MMP-9 (C and D). Cell viability was measured by using an MTT assay (A, 100% viability with untreated control). Bar graphs for viability (mean ± SEM, n = 5) was expressed as percent cell survival. RPE cell lysates were subject to western blot analysis using a primary antibody of ZO-1, occludin-1, MMP-2, MMP-9 and β-actin for an internal control (B–D). The bar graphs (mean ± SEM, n = 3) represent quantitative results of blot bands. * Values in respective bar graphs indicate a significant difference at p < 0.05. The mRNA transcriptional levels of ZO-1 and MMP-2 were measured using RT-PCR analysis. β-Actin was used as a housekeeping gene for the co-amplification with ZO-1 and MMP-2 (E).