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. Author manuscript; available in PMC: 2020 Oct 31.
Published in final edited form as: World Neurosurg. 2015 Jun 11;84(5):1256–66.e1. doi: 10.1016/j.wneu.2015.05.076

Figure 3.

Figure 3.

Immunocytochemical analysis. Three clones were analyzed with immunocytochemistry for pluripotency. Clone 9 shows that it expresses the pluripotency markers OCT4 (A) and SSEA-4 (B), 4′,6-diamidino-2-phenylindole (DAPI) (C). The clone also expresses SOX2 (D) and TRA-1–60 (E), DAPI (F). The pluripotency transcription factor Nanog is expressed as well (G), DAPI (H). Scale bars, 10 μm. OCT4, octamer-binding transcription factor 4; SOX2, (sex-determining region Y)-box 2; SSEA-4, stage-specific embryonic antigen 4; TRA-1–60, tumor rejection antigen 1–60.