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. Author manuscript; available in PMC: 2020 Oct 31.
Published in final edited form as: World Neurosurg. 2015 Jun 11;84(5):1256–66.e1. doi: 10.1016/j.wneu.2015.05.076

Figure 7.

Figure 7.

Targeted neural differentiation. After 17 days in neuronal differentiation medium, induced pluripotent stem cell clone 9 forms large (A; scale bar, 50 μm; B, magnification of A; scale bar, 10 μm) neural rosettes. Panel C (scale bar: 10 μm) shows a radial arrangement of elongated columnar cells, which is the morphologic characteristic of a neural rosette. After 19 days, differentiated cells express the neuronal marker TuJ-1 (D; green: TuJ-1; blue: 4′,6-diamidino-2-phenylindole; scale bar, 10 μm). Targeted differentiation leads to the formation of neuronal precursors, which co-express TuJ-1 (E, green) and Sox1 (F, red); TuJ-1/SOX1 (G; scale bar, 10 μm).