Figure 6.

Effect of AuNP concentration on encapsulant release. (A) The fluorescence intensity inside of an individual vesicle is measured as a function of time following a single 532 nm, fs pulse (144 nJ). Each line represents the fluorescence decay in an ROI (3–4 replicates +/− SEM) with varying concentrations of AuNPs encapsulated within the polymersome membrane. (B) Poration leading to structural instability. A polymersome loaded with 0.066% (w/v) AuNPs is subjected to a single fs pulse (144 nJ). Upon irradiation, a large pore can be observed. The vesicle remains intact for 10–20 s following the pulse until the vesicle structure is compromised after approximately 50 s.