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. 2020 Oct 16;13(10):315. doi: 10.3390/ph13100315

Figure 1.

Figure 1

Metformin blocks the nerve growth factor (NGF)-mediated effects on c-MYC in ovarian cells. Ovarian cells were treated with metformin 10 mM for 48 h and/or NGF 100 ng/mL or 150 ng/mL (A2780/human ovarian surface epithelial HOSE cells and SKOV3 cells, respectively) for 24 h or the last 2 h. (A) Representative Images of c-MYC immunodetection in HOSE cells with semi-quantification analysis. Bar = 100 µm. Lower right inserts: 400× magnification. Upper right insert: negative control (cells without primary antibody). N = 4 independent experiments (8 images were evaluated per experiment). (B,C) Western blots of c-MYC in A2780 and SKOV3 cells. (D,E) Gen-reporter assays to evaluate MYC transcriptional activity in the epithelial ovarian cancer (EOC) cells A2780 and SKOV3. N = 4 independent experiments. * p < 0.05; ** p < 0.01 and *** p < 0.001. Statistical analysis: Kruskal–Wallis test and Dunn’s post-test. B: basal condition (without stimuli), N: NGF, M: metformin treatment. Results are expressed as the mean ± standard error of the mean (SEM).