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. 2020 Oct 16;13(10):315. doi: 10.3390/ph13100315

Figure 3.

Figure 3

Metformin decreases the NGF-dependent increase in survivin expression in ovarian cells. Ovarian cells were treated with metformin 10 mM for 48 h and/or NGF 100 ng/mL or 150 ng/mL (A2780/HOSE cells and SKOV3 cells, respectively) for 24 h or the last 2 h. Then, survivin mRNA was detected in the ovarian cell lines HOSE, A2780 and SKOV3 by RT-PCR and protein levels were determined by Western blotting. (A–C) mRNA levels of survivin in the ovarian cell lines following metformin and NGF treatment. (D–F) Western blots of survivin in ovarian cell lines. N = 4 experiments per condition. * p < 0.05; ** p < 0.01 as indicated or with respect to basal condition. Statistical analysis: Kruskal–Wallis and Dunn´s post-test. † p < 0.05 with respect to basal condition or where indicated using the Mann–Whitney test. B: basal condition (without stimuli), N: NGF, M: metformin treatment. Results are expressed as the mean ± standard error of the mean (SEM).