Figure 5.

Metformin increases the expression of miR-145 and miR-23b in ovarian cell lines. Ovarian cells were treated with metformin (10 mM for 48 h) and NGF (100 or 150 ng/mL, last 3 h) to detect miR expression. miR-23b and miR-145 were quantified in HOSE, A2780 or SKOV3 cells by qPCR. N = 4 or more independent experiments in duplicate. * p < 0.05; ** p < 0.01 and *** p < 0.001 as indicated or with respect to basal conditions. Statistical analysis: Kruskal–Wallis and Dunn´s post-test. ^† p < 0.05 and ^†† p < 0.01 with respect to basal conditions or where indicated using the Mann–Whitney test. B: basal condition (without stimuli), N: NGF, M: metformin treatment. Results are expressed as the mean ± standard error of the mean (SEM).