Table 2.
Antibodies Used.
| Target | Host | Vendor (Location) | Catalog Number | Conjugation |
|---|---|---|---|---|
| Primary Antibodies | ||||
| PEG | Rabbit | Abcam | Ab51257 | N/A |
| Rat CD3 | Mouse | Becton Dickinson | 557030 | Allophycocyanin |
| Rat CD3 | Mouse | Becton Dickinson | 550295 | N/A |
| Rat CD4 | Mouse | Biolegend | 201516 | Phycoerythrin-cyanine-7 |
| Rat CD8 | Mouse | Becton Dickinson | 554857 | Phycoerythrin |
| Rat CD25 | Mouse | Becton Dickinson | 554865 | Fluorescein isothyocyanate |
| Rat CD45RC | Mouse | Invitrogen | MA5-17458 | Biotin |
| Rat/human podoplanin | Mouse | Abcam | Ab10288 | N/A |
| Secondary Antibodies | ||||
| Rabbit IgG | Goat | Life Technologies | P-10994 | Pacific Blue |
| Rabbit IgG | Horse | Vector Labs | MP-7401-50 | Horseradish peroxidase |
| Mouse IgG | Horse | Vector Labs | AP-2000-1 | Alkaline phosphatase |
| Streptavidin | N/A | Invitrogen | S32351 | Alexa Fluor 405 |