Figure 3.

SK-MEL-28 and A375 invasion is reduced by direct CAXII inhibition under hypoxia. (A) Modified Boyden chamber invasion assays after pretreatment and 24 h treatment under hypoxia (10× magnification). Data are presented as the means and standard errors of the mean of three independent experiments performed in triplicates. Acetazolamide (AAZ) was used as control. (B) Phosphorylated focal adhesion kinase (phFAK) protein expressions after 24 h treatment with C-7 and C-10 under hypoxia as determined by western blot analyses. Data are presented as the means and standard errors of the mean of three independent experiments. Β-actin was used as loading control. (C) Matrix metalloproteinase 9 (MMP-9) enzymatic activity after 24 h treatment upon hypoxia as determined by zymography. Data are presented as the means and standard errors of the mean of three independent experiments. (D) CAXII protein expression in SK-MEL-28 after 24 h treatment with C-7 and C-10 under hypoxia as determined by immunofluorescence microscopy analyses. (25× objective 50× magnification). Data are presented as the means and standard errors of the mean of three independent experiments. * p ≤ 0.05, ** p ≤ 0.01 indicate statistically significant differences. The uncropped blots of Figure 3B is shown in Figure S3.