Table 2.
Motility and velocity parameters of 4 h chilled sperm using different concentrations of canine amniotic membrane-derived mesenchymal stem cells CM.
| Concentration of CM (%) | Motility (%) | Viability (%) | 1 VCL (µm/s) | VSL (µm/s) | VAP (µm/s) | LIN (%) | STR (%) | ALH (µm) |
|---|---|---|---|---|---|---|---|---|
| 0 | 67.3 ± 2.5 b | 80.8 ± 2.0 b | 74.2 ± 4.4 b | 21.4 ± 1.3 | 45.8 ± 2.0 | 29.0 ± 1.3 | 47.4 ± 1.7 | 4.1 ± 0.1 b |
| 5 | 72.4 ± 2.5 b | 83.9 ± 2.8 ab | 75.4 ± 6.7 ab | 20.4 ± 1.0 | 46.8 ± 3.1 | 29.0 ± 1.5 | 44.1 ± 1.4 | 4.2 ± 0.3 b |
| 10 | 79.2 ± 2.6 a | 90.1 ± 2.8 a | 87.2 ± 8.1 a | 23.8 ± 1.8 | 54.0 ± 4.0 | 31.2 ± 1.6 | 44.5 ± 1.2 | 4.8 ± 0.3 a |
| 15 | 72.1 ± 3.9 b | 86.8 ± 3.5 ab | 75.4 ± 6.7 ab | 24.6 ± 3.7 | 46.7 ± 4.0 | 31.6 ± 2.1 | 46.4 ± 2.3 | 4.1 ± 0.3 b |
1 VCL, average curvilinear velocity; VSL, straight-line velocity; VAP, average path velocity; LIN, linearity (average ratio of VSL/VCL); STR, straightness (average value of the ratio VSL/VAP); ALH, amplitude of lateral head. All results show means ± SEM. Values within marked with the letters “a” or “b” are significantly different (p < 0.05, n = 4).