Table 1.
Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [15]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [15].
| Internal Number (MHI) | Other Designation | Source | Origin | panC Type | cry | nhe | hbl | ces | cytK1 | cytK2 | NheB * | Toxicity ** |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 271 | B. thuringiensis Berliner 1915, DSM 6029 | Animal tissue | A | IV | 1 | + | + | − | − | + | High | High |
| 2873 | B. thuringiensis Berliner 1915, ATCC® 10792™, DSM 2046 | Mediterranean flour moth | A | IV | 1 | + | + | − | − | + | High | High |
| 2882 | B. thuringiensis ssp. israelensis, BGSC 4Q2 (HD500) | Culicidae larva | A | IV | 4 | + | + | − | − | + | High | Medium |
| 3370 | B. thuringiensis Bt 407 | Ephestia kuehniella larva; Cry- | A | IV | − | + | + | − | − | + | High | High |
| 2880 | B. thuringiensis WSBC 28025 | Vegetables for infant food | F | IV | 1, 2 | + | + | − | − | + | Medium | High |
| 3163 | B. thuringiensis | Ready-to-eat salad 1 | F | IV | 1, 2 | + | + | − | − | + | High | High |
| 3164 | B. thuringiensis | Ready-to-eat salad 2 | F | IV | 1, 2 | + | + | − | − | + | Medium | High |
| 3189 | B. thuringiensis | Ready-to-eat salad 3 | F | IV | 1, 2 | + | + | − | − | + | Low | Medium |
| 3190 | B. thuringensis | Ready-to-eat salad 4 | F | IV | 1, 2 | + | + | − | − | + | Low | Medium |
| 3191 | B. thuringiensis | Ready-to-eat salad 5 | F | IV | 1 | + | + | − | − | + | Low | High |
| 2878 | B. thuringiensis ssp. tenebrionis | Undefined biopesticide | P | IV | 3 | + | + | − | − | − | Low | Medium |
| 3186 | B. thuringiensis ssp. aizawai | Biopesticide granulate 1 | P | IV | 1, 2 | + | + | − | − | + | Low | High |
| 3187 | B. thuringiensis ssp. aizawai | Biopesticide granulate 1 | P | IV | 1, 2 | + | + | − | − | + | Low | Medium |
| 3188 | B. thuringensis | Isolate from Biopesticide 1 | P | IV | 1, 2 | + | + | − | − | + | Low | High |
| 3240 | B. thuringensis ssp. kurstaki | Biopesticide 2 | P | IV | 1, 2 | + | + | − | − | + | Medium | High |
| 3241 | B. thuringiensis ssp. tenebrionis | Biopesticide 3, solids | P | IV | 3 | + | + | − | − | − | Medium | Low |
| 3369 | B. thuringiensis ssp. aizawai | Biopesticide 4 | P | IV | 1, 2 | + | + | − | − | + | Low | High |
| 2874 | B. thuringiensis ssp. kurstaki, PO1 | Soil | S | IV | 1 | + | + | − | − | + | High | Medium |
| 2875 | B. thuringiensis ssp. kurstaki, PO6 | Soil | S | IV | 1 | + | + | − | − | + | High | High |
| 2876 | B. thuringiensis ssp. kurstaki, PO10 | Soil | S | IV | 1 | + | + | − | − | + | Medium | High |
| 2877 | B. thuringiensis ssp. kurstaki, PO14 | Soil | S | IV | 1 | + | + | − | − | + | High | High |
| 2870 | B. thuringiensis ssp. entomocidus bv. subtoxicus, IEBC-T06A001 | Canada | U | IV | 1, 2 | + | + | − | − | + | Medium | Low |
| 2871 | B. thuringiensis | Undefined | U | IV | 1, 2 | + | + | − | − | + | Medium | Medium |
| 2872 | B. thuringiensis HER 1404 | Undefined | U | IV | 1, 2 | + | + | − | − | + | Medium | Medium |