Table 1.

Proportion of PCR mix.

Component	Volume per 10 μL Reaction
SsoAdvanced™ Universal SYBR® Green Supermix	5 μL
Forward and reverse primers	1 μL (0.8 μM)
DNA template	2 μL (0.04–0.015 ×10−4)
Nuclease-free water	2 μL