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Histological and immunohistochemical analysis of frozen–thawed ovarian tissue graft. Hematoxylin/eosin staining (A) (magnification ×40) and (B) (magnification ×100): (a) NOD.SCID mouse skin; (b) hair follicles; (c) fat follicles; (d) intermediate zone—de novo formatted connective tissue with neovascularization; (e) human ovarian tissue; (f) developing follicle. Ki–67 staining (C) (magnification ×40) and (D) (magnification ×100): no malignant transformation showed (expression < 5%).
