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Quality and quantity indicators of extracted RNA from frozen–thawed ovarian tissues. RIN—RNA integrity number; RT-qPCR—reverse transcription quantitative polymerase chain reaction.
| Indicator | Expected Value for RT-qPCR | Samples | ||||
|---|---|---|---|---|---|---|
| VIP–M-006 | VIP–M-007 | VIP–M-008 | VIP–M-011 | VIP–M-012 | ||
| Concentration (NanoDrop) | >21 ng/μL | 474 | 226 | 401 | 41 | 177 |
| Concentration (Agilent) | 343 | 145 | 257 | 23 | 120 | |
| A260/A280 (NanoDrop) | 1.9–2.1 | 2.00 | 2.04 | 2.02 | 1.96 | 2.06 |
| RIN (Agilent) | >6.5 | 6.80 | 6.60 | 7.20 | 7.60 | 7.70 |
