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. 2020 Oct 17;9(10):1486. doi: 10.3390/foods9101486

Figure 1.

Figure 1

C2C12 and B16-F10 cell growth. C2C12 (A,B) and B16-F10 (C,D) cell growth was measured after 24 h (A,C) and 48 h (B,D) of treatments with Phosphate Buffered Saline (PBS), as control, and different concentrations of O. vulgare plant extract (HCOE). Results expressed as percentage with respect to PBS represent the mean ± SD of four independent experiments (* p < 0.01; *** p < 0.001 vs. control). Proliferation curves of C2C12 (E) and B16-F10 (F) cells were generated counting, by a Neubauer modified chamber, the amount of alive cells after staining with Trypan Blue at 0, 24, and 48 h of treatments with HCOE. Results are indicated as the mean ± SD of four independent experiments. (* p < 0.05 vs. control) (G) Cell cycle analysis of B16-F10 after treatment, for 48 h, with 2, 4, 6, 8, and 10 mg/mL HCOE is shown. For each sample, the percentage amount of cells in every cycle phase (G0/G1, S, and G2/M) was measured by cytofluorimetric analysis. Results are indicated as mean ± SD of four independent experiments (* p < 0.05; ** p < 0.01; *** p < 0.001 vs. control).