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. 2020 Oct 17;9(10):1486. doi: 10.3390/foods9101486

Figure 6.

Figure 6

Immunofluorescence analysis. (A) The amount of γH2AX and 53BP1 foci detected in A375 cells treated with PBS (CNT), etoposide (ETO), 10 mg/mL HCOE (HCOE10), triethylenetetramine (TETA) or HCOE10+TETA were counted. Results are expressed as mean of foci per cell ± SD of three independent experiments (* p < 0.05; ** p < 0.01; *** p < 0.001 vs. control). (B) Representative immunofluorescence images of A375 cells treated with PBS (CNT) (a–d), etoposide (ETO) (e–h), 10 mg/mL HCOE (HCOE10) (i–l), triethylenetetramine (TETA) (m–p), or HCOE10+TETA (q–t) are shown. γH2AX foci are in green, 53BP1 foci are in red, while nuclei were stained in blue with DAPI. Merged images are also reported. The white bars indicate 15 μm.