Table 3.
Cytotoxicity analysis. The percentages of C2C12, B16-F10, and A375 dead cells identified by the Trypan Blue test after 24 and 48 h of HCOE treatment are reported. Data are reported as mean ± SD of four independent replicates. (* p < 0.05 vs. control).
| Cell Line | Treatment | Time | ||
|---|---|---|---|---|
| 24 h | 48 h | |||
| C2C12 | CNT (PBS) | 6.07 ± 0.80 | 4.61 ± 0.11 | |
| HCOE | (2 mg/mL) | 13.33 ± 0.41 | 11.67 ± 2.60 | |
| (4 mg/mL) | 13.16 ± 0.32 | 11.33 ± 3.43 | ||
| (6 mg/mL) | 18.86 ± 0.28 | 16.35 ± 0.80 | ||
| (8 mg/mL) | 20.25 ± 0.51 * | 19.78 ± 1.57 * | ||
| (10 mg/mL) | 20.67 ± 0.17 * | 21.25 ± 2.36 * | ||
| B16-F10 | CNT (PBS) | 1.86 ± 0.13 | 5.31 ± 0.26 | |
| HCOE | (2 mg/mL) | 14.90 ± 1.16 | 14.84 ± 1.17 | |
| (4 mg/mL) | 12.98 ± 2.38 | 26.62 ± 0.95 * | ||
| (6 mg/mL) | 24.36 ± 0.84 * | 33.29 ± 1.49 * | ||
| (8 mg/mL) | 37.30 ± 2.90 * | 39.45 ± 3.44 * | ||
| (10 mg/mL) | 42.00 ± 3.73 * | 44.75 ± 1.86 * | ||
| A375 | CNT (PBS) | 5.38 ± 1.09 | 5.00 ± 1.71 | |
| HCOE | (2 mg/mL) | 17.78 ± 1.38 | 13.10 ± 1.92 | |
| (4 mg/mL) | 16.82 ± 0.75 | 25.38 ± 2.96 * | ||
| (6 mg/mL) | 29.44 ± 1.17 * | 34.71 ± 1.86 * | ||
| (8 mg/mL) | 31.21 ± 3.81 * | 48.26 ± 0.77 * | ||
| (10 mg/mL) | 47.14 ± 1.73 * | 55.13 ± 1.47 * | ||