Table 1.
Results of in vitro cholinesterases inhibitory assay of 2-(hydroxy-(3-nitrophenyl)methyl)cyclopentanone.
| Samples | Conc. (µg/mL) | Acetylcholinesterase (AChE) | Buterylcholinesterase (BChE) | ||
|---|---|---|---|---|---|
| Inhibition (%) | IC50 µg/mL | Inhibition (%) | IC50 µg/mL | ||
| 3NCP | 07.8 15.6 |
16.17 ± 1.53 25.0 ± 5.0 * |
16.17 | 66.0 ± 2.65 224.0 ± 5.29 |
20.51 |
| 31.25 | 45.7 ± 4.04 | 44.3 ± 6.03 | |||
| 62.5 | 57.0 ± 3.0 | 55.0 ± 4.36 * | |||
| 125 | 65.3 ± 4.16 | 65.8 ± 1.89 | |||
| 250 | 72.3 ± 3.06 | 71.0 ± 5.57 | |||
| 500 | 77.0 ± 3.0 | 76.0 ± 1.73 | |||
| 1000 | 84.9 ± 5.0 | 86.3 ± 5.51 | |||
| Galantamine | 07.8 15.6 |
11.3 ± 1.6 36.0 ± 2.0 |
13.12 | 16.0 ± 2.0 31.7 ± 5.77 |
14.43 |
| 31.25 | 54.0 ± 3.0 | 57.0 ± 2.0 | |||
| 62.5 | 62.0 ± 2.0 | 70.0 ± 5.0 | |||
| 125 | 71.0 ± 2.2 | 75.0 ± 5.0 | |||
| 250 | 77.0 ± 3.0 | 80.0 ± 4.5 | |||
| 500 | 84.7 ± 2.0 | 88.0 ± 4.0 | |||
| 1000 | 88.7 ± 4.15 | 91.3 ± 2.31 | |||
It has concentration-dependent inhibitory properties against these enzymes. Inhibition was observed as 16.17% and 6% at 7.8 µg/mL, while it was 84.9% and 86.3% at 1000 µg/mL. The IC50 values of 16.17 and 20.51 µg/mL against AChE and BChE were determined. Similarly, galanthamine caused inhibition of AChE and BChE, which was 11.3 and 16% at 7.8 µg/mL, whereas it was 88.7% and 91.3% at 1000 µg/mL, with IC50 values of 13.12 and 14.43 µg/mL. * p < 0.005 compared to galanthamine treatment.