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. 2020 Oct 19;12(10):3193. doi: 10.3390/nu12103193

Figure 5.

Figure 5

A confirmed allergic response to whey protein reduced the percentages of spleen Th17 cells and tended to decrease H3 histone acetylation levels at the promoter responsible for transcription of RORγt. (A) The percentage of CD4+ T cells positive for RORγ(t) as assessed by flow cytometry. Data are presented as a scatter dot plot showing individual data points and mean. Open circles indicate the mice that were included in the histone modification analysis. Data of one mouse in the control group were excluded due to a technical error. Comparisons were performed with an unpaired t test. Histone acetylation levels at the promoter responsible for the transcription of RORγt (i.e., isoform 2) from the RAR-related orphan receptor γ encoding gene (Rorc) (B) and the interleukin 17A gene (Il17a) (C) in spleen-derived CD4+ T cells as determined by chromatin immunoprecipitation followed by qPCR. Data are presented as scatter dot plots showing individual data points and median. Comparisons were performed with a Mann–Whitney U test. * p < 0.05; p-values ≥ 0.05 and <0.2 are shown as numbers.