Figure 1.

Vaccination regimen schedules. Groups of mice were immunised with a placebo Control or BCG, delivered by either the Intradermal or Airway route. Six weeks later, corresponding groups of mice received a single boost immunisation with adenovirus expressing TB10.4 by the airway route (Ad). Four weeks after the boost for equivalent groups of mice, either the spleen and lung cells were isolated for immunological assessment or mice were challenged with ~ 200 CFU of M. bovis by the intranasal route. Bacterial burden in the spleen and lungs of challenged mice was then assessed both 4 and 8 weeks later.