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. 2020 Oct 30;10:18759. doi: 10.1038/s41598-020-75990-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative RT-PCR analyses of genes possibly involved the Fe partitioning (VvYSL1a, VvYSL1b and VvYSL3) in grapevine leaves. (A) Assessment of VvYSL1a gene expression in Cabernet Sauvignon and Cabernet Volos, both non-inoculated and inoculated with P. viticola. (B) Assessment of VvYSL1a gene expression in Sauvignon Blanc and Sauvignon Kretos, both non-inoculated and inoculated with P. viticola. (C) Assessment of VvYSL1b gene expression in Cabernet Sauvignon and Cabernet Volos, both non-inoculated and inoculated with P. viticola. (D )Assessment of VvYSL1b gene expression in Sauvignon Blanc and Sauvignon Kretos, both non-inoculated and inoculated with P. viticola. (E) Assessment of VvYSL3 gene expression in Cabernet Sauvignon and Cabernet Volos, both non-inoculated and inoculated with P. viticola. (F) Assessment of VvYSL3 gene expression in Sauvignon Blanc and Sauvignon Kretos, both non-inoculated and inoculated with P. viticola. The data were normalised to the tubulin and EF1α housekeeping gene. The relative expression ratios were calculated using untreated parental genotypes as a calibrator sample. The values reported are means ± SE of three biological replicates. Different letters indicate significant differences according to one-way ANOVA with Tukey post hoc tests (p < 0.05).