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. 2020 Oct 30;11(10):936. doi: 10.1038/s41419-020-03134-0

Fig. 1. The endogenous expression of MIAT, and effect of MIAT on BTB permeability were detected in vitro.

Fig. 1

a Relative expression of MIAT in ECs and GECs were detected by qRT-PCR. GAPDH was used as an endogenous control. Data represent means ± SD (n = 3, each group). *P < 0.05 vs. ECs group. b TEER values of GECs were expressed as Ω cm2. Data represent mean ± SD (n = 3, each group). *P < 0.05 vs. MIAT(+) NC group. #P < 0.05 vs. MIAT(−) NC group. &&P < 0.01 vs. Control. c HRP flux was calculated as pmol/cm2/h. Data represent mean ± SD (n = 3, each group). *P < 0.05 vs. MIAT(+) NC group. ##P < 0.01 vs. MIAT(−) NC group. &P < 0.05 and &&P < 0.01 vs. Control. d, e The relative expression level of miR-140-3p and ZAK in GECs was mesured by qRT-PCR. U6 RNA level was used as an internal control for miR-140-3p, and GAPDH was used as an endogenous control for ZAK and NFκB-p65. Data represent mean ± SD (n = 3, each group). *P < 0.05 vs. MIAT(+) NC group. #P < 0.05 and ##P < 0.01 vs. MIAT(−) NC group. f The protein expression of ZAK was detected by western blot. g, h The protein expression of NFκB-p65 was shown by western blot. i Western blot analysis of TJ-associated proteins ZO-1, occludin, and claudin-5 in GECs. For panels f–i, GAPDH protein levels were used as an endogenous control. Data represent mean ± SD (n = 3, each group). *P < 0.05 and **P < 0.01 vs. MIAT(+) NC group. #P < 0.05 and ##P < 0.01 vs. MIAT(−) NC group. j Immunofluorescent localization of ZO-1, occludin, and claudin-5 in GECs. ZO-1 (red), occludin (red), and claudin-5 (red) were, respectively, labeled with fluorescent secondary antibody and nuclei were labeled with DAPI. Images were representative of five independent experiments. Scale bar = 20 µm.