Skip to main content
. 2020 Oct 30;11(10):936. doi: 10.1038/s41419-020-03134-0

Fig. 6. NFκB-p65 bound to the promoters of TJ-associated proteins in GECs of BTB.

Fig. 6

a Schematic representation of the human ZO-1 promoter regions in 3000 bp upstream of the transcription start site (TSS, designated as +1). Chip PCR products for binding sites and upstream regions not expected to associate with NFκB-p65 were amplified by PCR using their specific primers. Images were representative of independent Chip experiments. b Dual luciferase reporter assays were performed to determine the binding sites of NFκB-p65 and ZO-1 in HEK-293T cells. Data represent mean ± SD (n = 3, each). *p < 0.05 vs. NFκB-p65(+) NC group. c Schematic representation of the human occludin promoter regions in 3000 bp upstream of the transcription start site (TSS, designated as +1). Chip PCR products for binding sites and upstream regions not expected to associate with NFκB-p65 were amplified by PCR using their specific primers. Images were representative of independent Chip experiments. d Dual luciferase reporter assays were performed to determine the binding sites of NFκB-p65 and occludin in HEK-293T cells. Data represent mean ± SD (n = 3, each). *p < 0.05 vs. NFκB-p65(+) NC group. e Schematic representation of the human claudin-5 promoter regions in 3000 bp upstream of the transcription start site (TSS, designated as +1). Chip PCR products for binding sites and upstream regions not expected to associate with NFκB-p65 were amplified by PCR using their specific primers. Images were representative of independent Chip experiments. f The putative NFκB-p65 binding sites in the claudin-5 and designed mutant sequences are presented. Relative luciferase activity was performed by dual-luciferase reporter assays. Data represented as mean ± SD (n = 3). **P < 0.01 vs. NFκB-p65(+) NC group.