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. 2020 Oct 30;10:18746. doi: 10.1038/s41598-020-75264-6

Figure 1.

Figure 1

p39-associated Cdk5 activity is important for dendritic development. (a–d) Cdk5 activity is required for proper dendritic development. Rat hippocampal neurons at 7 days in vitro (DIV) were transfected with vector control or the indicated Cdk5 cDNA constructs, and dendritic morphology was examined at 14 DIV. (a) Representative images of wild-type neurons (Cdk5-WT) and Cdk5 activity-deficient neurons (Cdk5-DN or Cdk5-Y15F). Dendritic complexity was analyzed by quantifying total dendrite number (b) and total dendrite length (c), and Sholl analysis (d). Scale bar: 20 μm; n = 17–46 neurons from 3 independent experiments; **p < 0.01, ***p < 0.001 versus Mock; #p < 0.05, ##p < 0.01, ###p < 0.001 versus Cdk5-WT; Kruskal–Wallis one-way ANOVA followed by Dunn’s test. (e–h) Reduced dendritic complexity in Cdk5-, p35-, or p39-knockdown neurons. Rat hippocampal neurons at 7 DIV were transfected with vector control or shRNA targeting Cdk5, p35, p39, or p39 together with RNAi-resistant p39 mutant (Rr_p39). Dendritic morphology was examined at 14 DIV. Representative images (e), quantification of total dendrite number (f) and total dendrite length (g), and Sholl analysis (h). Scale bar: 20 µm; n = 30–35 neurons from 3 independent experiments; ***p < 0.001 versus Vector; ###p < 0.001 versus sh_Cdk5; †p < 0.05 versus sh_p39; Kruskal–Wallis one-way ANOVA followed by Dunn’s test.