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. 2020 Oct 30;10:18746. doi: 10.1038/s41598-020-75264-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphoproteomic analysis of the hippocampi of 1-month-old p39^+/+ and p39^−/− mice. (a) Workflow of phosphoproteomic analyses between p39^+/+ and p39^−/− hippocampi. (b) Gene Ontology (GO) analysis according to the PANTHER classification system revealed the protein classification of differentially phosphorylated proteins between p39^+/+ and p39^−/− mouse hippocampi. (c) Ingenuity pathway analysis (IPA) of the biofunctions of differentially regulated phosphoproteins in p39^+/+ and p39^−/− mouse hippocampi. The activation of biofunctions based on the differentially regulated phosphoproteins between p39^+/+ and p39^−/− hippocampi was determined by the z-score algorithm with a criterion of p < 0.05 (i.e., − log₁₀ ≥ 1.3; black dots) using Fisher’s exact test. (d) IPA of the canonical pathways of differentially regulated phosphoproteins between p39^+/+ and p39^−/− mouse hippocampi. The activation of canonical pathways based on the differentially phosphorylated protein expression between p39^+/+ and p39^−/− hippocampi was determined by the z-score algorithm with a criterion of p < 0.05 (i.e., − log₁₀ ≥ 1.3; black dots) using Fisher’s exact test.