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. 2020 Oct 30;6:46. doi: 10.1038/s41522-020-00154-8

Fig. 2. An overview of annotated genes in selected prophage regions and their essentiality classification.

Fig. 2

MPAO1-unique essential genes are shown in dark blue, general essential MPAO1 genes with a red arrow outline. a Genes located in prophage region 2 of PAO1-UW (gray), the corresponding inverted region in strain MPAO1 (light blue arrows in middle), and the prophage region 3 (light blue arrows on top) unique to MPAO1 are shown (not drawn to scale), the genomic positions of their boundaries (5′–3′) and flanking tRNAs. Genes connected by lines are orthologous to each other based on comparative genomics combined with a Blast analysis. b Transposon insertions in selected genes of prophage region 3 of MPAO1. Insertion frequencies in six genes are shown using data mapped from the LB-1 (3 replicates), LB-2 (2 replicates), and LB-3 (1 sample) Tn-seq libraries. Non-essential genes (based on a data set of 577 genes essential in one of three primary growth conditions) are shown in light blue.