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. 2020 Oct 30;6:46. doi: 10.1038/s41522-020-00154-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Differential protein abundance between MPAO1 mid-exponential planktonic cells and 72 h biofilms. Selected significantly upregulated proteins (red dots) known to have a role in biofilm formation/growth are labeled, proteins downregulated in planktonic growth are shown in blue. Red triangles denote proteins encoded by genes missed in the MPAO1/P1 genome. b Proteogenomic expression evidence for a longer protein than annotated by RefSeq: the Prodigal predicted protein MPAO1prod_16460 (gray arrow; 447 aa; amino acid) is 44 aa longer than the RefSeq annotated MPAO1_08365 and encodes a glutamine synthetase (blue arrow; 413 aa). The NH-terminal extension is supported by 1 peptide (red) with seven PSMs and harbors a 40 aa longer glutamine synthetase N-terminal domain compared to the RefSeq protein. c Proteogenomic expression evidence for a single nucleotide insertion (red) in the MPAO1_25975 gene (blue arrow) compared to its PAO1 homolog PA4875 (annotated as pseudogene; gray open arrow). The change is supported by peptide evidence (1 red bar).