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. 2020 Oct 19;9(10):1494. doi: 10.3390/foods9101494

Figure 2.

Figure 2

The effect of NV-LJ3402 on metabolic gene expression in the WATs of HFD-fed mice and 3T3-L1 adipocytes. After 14 weeks of NV-LJ3402 administration to HFD mice or 48-h treatment of day 6 3T3-L1 adipocytes with NV-LJ3402-CM or control bacterial culture medium (con), the expression of genes coding for proteins involved in lipogenesis and energy dissipation in WATs (a) and 3T3-L1 adipocytes (b) were determined by Real time-quantitative PCR (RT-qPCR), as indicated. (c) 3T3-L1 adipocytes were treated with NV-LJ3402-CM for different time periods, as indicated. Lipid accumulation in 3T3-L1 adipocytes was analyzed on day 8 by Oil Red O staining. All data are expressed as the mean ± S.E.M. The experiments using WATs were performed with n = 6–7 per group, and the results using 3T3-L1 adipocytes are from three independent experiments. HFD vs. HFD+NV-LJ3402, con vs. NV-LJ3402-CM, * p < 0.05, ** p < 0.01, *** p < 0.001.