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. 2020 Oct 19;12(10):3195. doi: 10.3390/nu12103195

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of AF on C2C12 myoblast treated with conditioned media (CM) from CT-26 cells. Protein levels of MAFbx, MuRF-1, and MyH were measured after treating WAF-CM (A). The relative protein expression levels of MAFbx, MuRF-1, and MyH (B). Protein levels of MAFbx, MuRF-1, and MyH were measured after treating with EtAF-CM (C). The relative protein expression levels (D). GAPDH was endogenous control. mRNA levels of MAFbx and MuRF-1 were measured (E). GAPDH was used as an endogenous control. The results are from three independent experiments. All values are mean ± SD. # p < 0.05, significantly different from control group; * p < 0.05, significantly different from CM group.