Effects of AF on the differentiation of 3T3-L1 cell treated with conditioned media (CM) from CT-26 cells. Oil Red O staining images (200× magnification) (A). Stained Oil Red O solution was resolved and measured (B). Cell viability was determined after incubating with CM treated with or without WAF-CM and EtAF-CM (C). The protein and mRNA levels of PPARγ and C/EBPα in 3T3-L1 cells after fully differentiation (D,E). GAPDH was used as an endogenous control. mRNA levels of FAS, LPL, and aP2 were measured (F). The results are from three independent experiments. All values are mean ± SD. # p < 0.05, significantly different from control group; * p < 0.05, significantly different from CM group.