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. Author manuscript; available in PMC: 2021 Jan 24.
Published in final edited form as: Nature. 2020 Jul 24;586(7827):113–119. doi: 10.1038/s41586-020-2577-1

Figure ED8. In vitro protease assay on SARS-CoV-2 papain-like protease (PLpro) and main protease (Mpro).

Figure ED8.

Purified SARS-CoV-2 Mpro (A) and SARS-CoV-2 PLpro (B) enzymes were incubated with varying concentrations of each compound, ranging from 1 to 50 μM. Activity of purified SARS-CoV-2 Mpro and SARS-CoV-2 PLpro enzymes was measured using the UIVT-3 peptide substrate (HiLyte Fluor488TM-ESATLQSGLRKAK-QXL520TM-NH2) and the peptide substrate Arg-Leu-Arg-Gly-Gly-AMC (RLRGG-AMC) respectively. Enzyme activity in the absence (zero percent inhibition control) and presence of compounds were used to calculate the percent inhibition at each compound concentration. Data are presented as mean ±SD for n=3 independent experiments.