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. 2020 Oct 27;13:10867–10876. doi: 10.2147/OTT.S266449

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© 2020 Han et al.

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YAP promotes GC cell proliferation and migration in vitro.

Notes: (A) YAP knockdown inhibited the proliferation of AGS cells by colony formation assays. Quantitative analysis results were presented as the mean ± SEM (n = 3). (B) YAP overexpression promoted the proliferation of MKN45 cells by colony formation assays. Quantitative analysis results were presented as the mean ± SEM (n = 3). (C) YAP knockdown inhibited the migration of AGS cells by transwell migration assays. Quantitative analysis results were presented as the mean ± SEM (n = 3). (D) YAP overexpression promoted the migration of MKN45 cells by transwell migration assays. Quantitative analysis results were presented as the mean ± SEM (n = 3). (E) E-cadherin and Vimentin expression in MGC803 and AGS GC cells stably transfected with control-shRNA (NC) or shRNA against YAP (KD) tested using Western blot analysis. The bands were quantified and showed as the mean ± SEM of triplicate determination from three independent experiments. (F) E-cadherin and Vimentin expression in SGC7901 and MKN45 GC cells stably transfected with empty vector (VEC) or plasmid encoding human YAP (OE) determined by Western blotting. The bands were quantified and showed as the mean ± SEM of triplicate determination from three independent experiments. GAPDH as a loading control. Statistical significance was analyzed by a two-tailed, unpaired Student’s t-test. **P < 0.01.

Abbreviations: GC, gastric cancer; NC, control-shRNA; KD, shRNA against YAP; VEC, empty vector; OE, plasmid encoding human YAP; SEM, standard error of the mean; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.