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. 2020 Jul 22;94(11):3831–3846. doi: 10.1007/s00204-020-02856-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Differentiation efficiency and gene expression analysis for three cell lines. a All three cell lines (BIONi010-C, IMR90-1, and IMR90-4) were differentiated into cardiomyocytes and beating embryoid bodies (EBs) counted on day 6 and day 7. Thirty-two EBs were counted per experiment. Shown in percent of all counted EBs, mean and SEM of three differentiation experiments with each cell line. b All three cell lines (BIONi010-C, IMR90-1, and IMR90-4) were differentiated into cardiomyocytes and samples taken every day of differentiation. Mean and SEM of ≥ 2 differentiation experiments for each cell line. Stars indicate significant differences between cell lines calculated with a two-way ANOVA. Columns without stars do not differ significantly. n = 4 (BIONi010-C), n = 2 (IMR90-1 and IMR90-4)