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. 2020 Oct 30;10:18768. doi: 10.1038/s41598-020-75542-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Figure. 2 — Effects of BCAA supplementation on HF-induced fat accumulation in the liver (A) and hepatic lipogenesis-related protein expression of FAS (B) and ACC (C). Representative adipophilin immunostaining of the liver (A). Values are expressed as the means ± S.D. (B, C). *p < 0.05 between the groups connected by a line. Scale bar = 20 μm. A representative band of each group was trimmed. Full-length gels are presented in Supplementary figure. S9. ST, fed a standard diet; ST-BCAA, fed a ST with added BCAAs; HF, fed a high-fat diet; and HF-BCAA, fed a HF with added BCAAs.