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. 2020 Oct 30;10:18718. doi: 10.1038/s41598-020-75561-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Conditions used for the dissociation of EVs from K8-peptide-immobilized magnetic beads. EVs bound to K8-peptide-immobilized magnetic beads were dissociated with SDS sample buffer or buffers with the indicated concentrations of NaCl. The released materials were analyzed by western blotting using anti-CD9 and CD63 antibodies. The materials released with 0.5 M NaCl were analyzed by western blotting using an anti-TSG101 antibody. Images of the full western blots are presented in Supplementary Fig. S3 (for TSG101), Fig. S5 (for CD9), and Fig. S6 (for CD63).