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. 2020 Nov 1;7:182. doi: 10.1038/s41438-020-00404-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Leaf shape in OE and wild-type CU2. Six successive leaves (from the left) were harvested from the top of the same plant. b PCR assay of the OE and CU2 lines. PCR targeted the CDS of the eGFP and HYP genes in the plasmid. M: DNA marker. c Semiquantitative PCR assay of the OE and CU2 leaves. d Fluorescence detection of the OE and CU2 roots. e The area ratio of leaves from OE to those from CU2 using six successive top leaves from the same plant representing different growth stages. Each point represents a value in the set of data, and the short black line represents the mean of this set of data. Five biological repeats were included per measurement. f Abnormal leaves (‘butterfly-shaped’) with seven primary veins extending from the petiole in the OE plant. Bar = 5 cm (a), 2 mm (d), and 3 cm (f)