Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 31;11(10):938. doi: 10.1038/s41419-020-03136-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — HCT116 and SW480 cells were treated with 0.7 μmol/L m-THPC in the presence or absence of 5 mmol/L NAC. NAC and m-THPC were added to the medium 2 and 8 h before irradiation, respectively, and then irradiated with a light dose of 3 J/cm². Followed by incubation without irradiation for 8 h, A, B Western blot analysis of MAP1LC3B-I, MAP1LC3B-II, and SQSTM1/p62 levels in HCT116 and SW480 cells. C The level of ROS production in HCT116 and SW480 cells was analyzed by flow cytometry to detect DCF fluorescence intensity. D, E GFP-MAP1LC3B puncta were observed by immunofluorescence using a laser scanning confocal microscope. F After 24 h of radiation, flow cytometry was performed to measure cell apoptosis with Annexin V-FITC and PI staining. All data are presented as the mean ± SD, n = 3. ^**P < 0.01, ^***P < 0.001.