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. 2020 Oct 31;11(10):938. doi: 10.1038/s41419-020-03136-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — HCT116 and SW480 cells were transfected with 50 nmol/L siATG5 or siATG7 for 48 h, respectively. After that, cells were treated with 0.7 μmol/L m-THPC for 8 h, and then irradiated with a light dose of 3 J/cm², followed by incubation without irradiation for 16 h. A–F Western blot analysis of ATG5, ATG7, MAP1LC3B-I, MAP1LC3B-II, and SQSTM1/p62 levels in HCT116 and SW480 cells. G, H Flow cytometry was performed to measure cell apoptosis. All data are presented as the mean ± SD, n = 3. ^*P < 0.05, ^**P < 0.01.