Figure 4.

Mfn2 siRNA inhibited mitochondrial Ca²⁺ overload during ER stress induced by TM. (a) Representative confocal microscopy images of fluorescence staining for Fluo-4 AM and DAPI. Scale bar, 20 μm. (b) Quantification of Fluo-4 AM fluorescence intensity in (a). (c) Representative confocal microscopy images of fluorescence staining for Rhod2 AM and MitoTracker Green. Scale bar, 20 μm. (d) Quantification of Rhod2 AM fluorescence intensity in (c). (e) Mitochondria Ca²⁺ proteins such as VDAC1 and MCU protein level in atrial myocytes detected by Western blot analysis. (f) Quantification of VDAC1 and MCU protein level in (e). Data are mean ± SEM, n = 4 independent experiments, ^#p < 0.01, ANOVA with Bonferroni posttest.