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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Cell Rep. 2020 May 26;31(8):107694. doi: 10.1016/j.celrep.2020.107694

Figure 5. Lnc-AdipoAR1 Knockdown Inhibits the Expression of Adipogenesis Markers.

Figure 5.

(A) RNA-seq expression of RP23–218F13.7, RP24–501G17.2, and lnc-AdipoAR1 (AC116511.4) in eWAT and iWAT during aging.

(B) Real-time PCR validations of RP23–218F13.7, RP24–501G17.2, and lnc-AdipoAR1 in eWAT and iWAT during aging. The expression of each gene was normalized to house-keeping gene Cyclophilin A (CPA). n = 5; error bars are mean ± SEM.

(C) The expression of lnc-AdipoAR1 across different tissues during aging.

(D) Real-time PCR analysis of lnc-AdipoAR1 during adipogenesis of 3T3-L1 adipocytes. The expression of lnc-AdipoAR1 was normalized to RPL-23. n = 4; error bars are mean ± SEM.

(E–G) lnc-AdipoAR1 was knocked down with ASOs in iWAT-derived adipocyte culture at day 0 (E), day 3 (F), or day 5 (G) during adipogenesis. RNA was harvested at indicated timing for real-time PCR to examine the expression of indicated adipocyte markers. Gene expression was normalized to RPL-23. n = 4 (E and F) and n = 3 (G); error bars are mean ± SEM, *p < 0.05, Student’s t test.