Fig. 3.

Overexpression of CXCR7 in A549 lung cancer cell line. a. The structure of CXCR7 overexpression vector. b. FACS analysis of CXCR7 protein expression in constructed A549-GFPLuc-CXCR7 overexpression lung cancer cell line. CXCR7 protein expression was evaluated by FACS analysis using PE-conjugated anti-CXCR7 antibody, and anti-IgG1 served as isotype control. Transduction of GFPLuc was used for report gene and firefly luciferase bioluminescence imaging of lung cancer animal model in vivo. c. Western blot was conducted to validate high-expressed CXCR7 in successful constructed A549-GFPLuc-CXCR7 overexpression lung cancer cell line. β-actin was used as a loading control