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. 2020 Oct 31;20:329. doi: 10.1186/s12866-020-01976-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effects of a mixture of acetic acid, propionic acid and butyric acid on INAC. INAC areas of wild-type A. oris MG1 and MG1 ΔfimA by live cells and with dead cells were observed in the control, with no acid; with 60 mM butyric acid treatment; mixture of 5 mM butyric acid, 15 mM propionic acid and 40 mM acetic acid treatment; and under the pH 4.7 condition prepared with HCl in mixture of 5 mM butyric acid and 15 mM propionic acid, and 20 mM butyric acid. The attached and colonized cells were stained using a LIVE/DEAD staining kit and were observed by CLSM at the edges of the wells. All CLSM images were obtained with a 10× objective. Images were further analysed to determine the live and dead cell areas in MG1 using ImageJ. Representative data from more than three independent experiments are presented in each panel