Table 1.

Comparison of Different Comet Analysis Methods

On-Chip Exposure CometChip	Off-Chip Exposure CometChip	SpheroidChip
Load single cell suspension directly before chemical exposure	Load single cell suspension after chemical exposure (~40 min lag)	Culture spheroids on chip ~2–3 days in advance, grow to diameter of microwell
20 min chemical treatment		30 min chemical treatment
Overnight lysis in high salt buffer and detergent
40 min alkaline unwinding step at high pH		1 hour alkaline unwinding at high pH
30 minute electrophoresis		1 hour electrophoresis
Image at 4X		Image at 2X