Table I.
Developmental rate of in vivo and IVF embryos at E3.5 and E4.5.
| Group | Number (%) | ||||||
|---|---|---|---|---|---|---|---|
| Mean (SD ± SEM) from each dam | |||||||
| Dam number | Embryo number | Morula | Early blastocyst | Mid blastocyst | Late blastocyst | Arrested (early cleavage) | |
| in vivo | 22 | 469 | 15 (3.2) | 40 (8.5) | 114 (24.3) | 295 (62.9) | 5 (1.1) |
| E3.5 | 0.71 (0.72 ± 0.15) | 1.9 (0.89 ± 0.19) | 5.43 (1.29 ± 0.27) | 14.05 (3.31 ± 0.7) | 0.24 (0.44 ± 0.09) | ||
|
| |||||||
| IVF | 40 | 10761 | 876 (81.4) | 0 | 0 | 0 | 200 (18.6) |
| E3.5 | 62.57 (38.14 ± 6.03) | 14.28 (5.69 ± 0.9) | |||||
| IVF | 109 (12.4)2 | 51 (5.8)2 | 83 (9.5)2 | 633 (72.3)2 | 0 | ||
| E4.5 | 7.78 (5.1 ± 0.81) | 3.64 (1.98 ± 0.31) | 5.92 (3.19 ± 0.5) | 45.21 (28.12 ± 4.45) | |||
Early blastocyst has a blastocoel volume less than half of the total embryo volume.
Mid blastocyst has a blastocoel volume equal to or larger than the total embryo volume.
Late blastocyst blastocoel fully expanded within the embryo whilst the zona pellucida (ZP) is thinning.
1
Number of 2-cell embryos cultured after IVF.
2
Per cent of morulae at E3.5.