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. 2020 Sep 10;8(5):390–398. doi: 10.1093/gastro/goaa049

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© The Author(s) 2020. Published by Oxford University Press and Sixth Affiliated Hospital of Sun Yat-sen University

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Programmed death-1 ligand 1 (PD-L1) expression is elevated in HepG2-SR and Huh7-SR cells, which acquire epithelial-mesenchymal transition (EMT) characteristics. (A) HepG2, Huh7 cells, and the sorafenib-resistant hepatocellular carcinoma (HCC) cells denoted as HepG2-SR and Huh7-SR are incubated with increasing doses of sorafenib for 2 days. Cell viability (%) was compared to that of the corresponding untreated cells. (B) and (C) Western-blot analysis of E-cadherin, N-cadherin, and PD-L1 protein expression in HepG2-SR, Huh7-SR cells, and their corresponding parental lines. Densitometry values for each protein are normalized to those of β-actin and shown below as the corresponding bands.