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. 2020 Nov 2;9:21. doi: 10.1186/s13619-020-00059-z

Fig. 3.

Fig. 3

Comparison of different culture methods for adult stem cells-derived organoids. In the WENR method, epithelial organoids are derived from tumor biopsies directly in Matrigel with cocktail growth factors, with long-term expansion but no tumor micro environment. In the air-liquid interphase (ALI) method, tumor biopsies are cultured in ALI in the entire tumor microenvironment as a cell suspension of all cell types, including immune cells and other non-epithelial cell types, but with limited expansion