Fig. 4.

Morphology of several types of human adult stem-cell organoids. a A schematic diagram showing the growth pattern of organoids. Typically, isolated cells or functional aggregates are embedded in extracellular matrix domes and cultured in media with essential niche factors. They gradually build tissue-like 3D structures within 1-2 weeks. b Bright-field image of a typical murine small intestinal organoid culture. c Bright-field image and HE staining image of typical human normal colon epithelium, adenoma and adenocarcinoma organoids