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. 2020 Oct 8;43(10):848–855. doi: 10.14348/molcells.2020.0135

Fig. 3. Disrupting SG assembly using salubrinal impedes neuronal differentiation.

Fig. 3

(A) hBM-MSCs treated with/without salubrinal were induced to differentiate into neurons for the indicated times, and total protein was extracted. p-eIF2α and NF-M expression levels were measured with their specific antibodies. (B) Differentiating cells were fixed and stained with G3BP (orange) and TIA-1 (green) antibodies. SG-positive cells are indicated by an arrowheads. Scale bars = 50 μm. (C) Nascent proteins were measured using the AHA labeling in differentiating hBM-MSCs treated with/without salubrinal.